BackgroundBromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated.The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting.These methods are more difficult and require more cleanup due to the radioactive material.An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.

Product

Formulation: Unstained mouse intestine sections, formalin-fixed, paraffin-embedded.Sections obtained from mice after treatement with BrdU.

ApplicationsOptimal concentration should be evaluated by serial dilutions.

StorageProduct should be stored at 4-8ºC.

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, refrigerate upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Exalpha提供用以临床免疫组化实验的抗体，和常规研究用的信号通路抗体，流式细胞用的CD markers，细胞增殖实验检测产品，Elisa试剂盒及用于药物发现用的GPCR膜

评估细胞周期进程在许多研究领域都非常重要，传统的方法是将[3H]胸腺嘧啶核苷掺入S期细胞，然后通过闪烁计数或放射自显影来定量[3H], 以此来检测细胞增殖，但该技术流程耗时且费力，还有许多限制因素比如同位素污染处理和设备昂贵等问题。