BackgroundThe reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:50 upwards; in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

SourceHighly purified Bence Jones lambda proteins isolated from pooled human urine.

ProductPurified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.

Product Form: HRP

Formulation: Purified monoclonal mouse IgG1 kappaconjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

SpecificityHorseradish peroxidase conjugated purified monoclonal Mouse antibody to Human Ig lambda chain, free and bound

Species Reactivity: The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

ApplicationsELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day.

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Exalpha提供用以临床免疫组化实验的抗体，和常规研究用的信号通路抗体，流式细胞用的CD markers，细胞增殖实验检测产品，Elisa试剂盒及用于药物发现用的GPCR膜

评估细胞周期进程在许多研究领域都非常重要，传统的方法是将[3H]胸腺嘧啶核苷掺入S期细胞，然后通过闪烁计数或放射自显影来定量[3H], 以此来检测细胞增殖，但该技术流程耗时且费力，还有许多限制因素比如同位素污染处理和设备昂贵等问题。