BackgroundImplicated in the control of cell fate decisions during development.Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.

Synonyms: Protein S171; Protein numb homolog

Source

Immunogen: Synthetic peptide derived from human Numb protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

ApplicationsAntibody can be used for Western blotting (1-5 µg/ml).Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Protein found in brain, liver, testis and epithelium

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References1. Susini, L., et al. "Siah-1 binds and regulates the function of Numb.; Proc. Natl. Acad. Sci. U.S.A. 98:15067-15072(2001).2. Verdi, J.M., et al. "Distinct human NUMB isoforms regulate differentiation vs. proliferation in the neuronal lineage.; Proc. Natl. Acad. Sci. U.S.A. 96:10472-10476(1999).

Protein Reference(s)

Database Name: UniProt

Accession number: P49757 (Human)

Species Accession: Human

Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf

Exalpha提供用以临床免疫组化实验的抗体，和常规研究用的信号通路抗体，流式细胞用的CD markers，细胞增殖实验检测产品，Elisa试剂盒及用于药物发现用的GPCR膜

评估细胞周期进程在许多研究领域都非常重要，传统的方法是将[3H]胸腺嘧啶核苷掺入S期细胞，然后通过闪烁计数或放射自显影来定量[3H], 以此来检测细胞增殖，但该技术流程耗时且费力，还有许多限制因素比如同位素污染处理和设备昂贵等问题。